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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Glia. 2011 May 4;59(7):1084–1093. doi: 10.1002/glia.21181

Figure 2. Astrocytic expression of dnSNARE leads to decreased tyrosine phosphorylation of Src kinase and NMDAR subunits.

Figure 2

(A) Somatosensory cortical slices were lysed and soluble material was immunoblotted with anti-Src and phospho-Src Y416 (p-Src)antibodies. The normalized amount of p-Src in nSNARE was significantly smaller than WT (n= 4). (B) NR2A was immunoprecipitated (IP) then probed with anti-p-Y antibody. In dnSNARE there was significantly reduced Y-phosphorylation of NR2A compared to WT (n=3). (C) Soluble material was immunoblotted with anti-NR2B and anti-NR2B phospho-NR2B Y1472 (p-NR2B) antibodies. dnSNARE led to reduced phosphorylation of Y1472 of the NR2B subunit (n=4). (D) Soluble material was immunoblotted with anti-GluR1 and phospho-GluR1 S845 (p-GluR1).p-GluR1. The normalized amount of p-GluR1 in dnSNARE was not difference from WT (n= 4). (E) Immunoblots of IP obtained with anti-NR2B antibody. Blots probed with anti-NR2B and anti-β adaptin antibodies. The amount of AP-2 in dnSNARE was normalized to WT. The amount of AP-2 in dnSNARE was significantly larger than in WT (n=4). Error bars, mean±SEM; * P<0.05.