Figure 2.

MAPK pathway inhibition by PD98059 during hepatocyte seeding after FCS and EGF stimulation. Hepatocytes were preincubated for 30 min with solvent (0.2% DMSO) (1, 2, 3, 4, 9, and 10) or inhibitor (75 μM PD98059) (6, 7, 12, and 13) before seeding and then cultured in absence (b; 1 and 2) or in presence of 10% FCS (3, 4, 6, and 7) or 50 ng/ml EGF (9, 10, 12, and 13) and analyzed for extension after 6 or 24 h. Bar, 150 μm. Solvent control (5 and 11) or PD98059 (8 and 14) were then removed after 24 h of exposure, and hepatocytes were observed 24 h later, i.e ,48 h after seeding. These data are representative of three different experiments.