Figure 5.

Inhibition of cell spreading and DNA replication by cytochalasin D treatment in EGF-stimulated hepatocytes. (A) Inhibition of spreading by cytochalasin D. Hepatocytes were stimulated at seeding by EGF in the presence or absence (control) of cytochalasin D at 1 μM and observed 24 h later. (B) Time course of [methyl-³H]thymidine incorporation into DNA in EGF-stimulated cells maintained (open bar) or not (black bar; solvent control) in the presence of cytochalasin D (1 μM) throughout the culture. (C) Reversion experiment in which cytochalasin D was removed after 48 h of treatment and DNA replication analyzed by thymidine incorporation at the indicated times after seeding. Labeled thymidine was added 4 h before cell harvesting. Values expressed in counts per minute per microgram of protein (prot) are means of duplicates from three different experiments.