Figure 4.

Expression of the PF6 transcript. (A) Northern blot loaded with total RNA isolated from wild-type, pf6 mutants, and a rescued pf6 strain before (0) and forty-five (45) min after deflagellation. This blot was probed with an RT-PCR product from within probe C (Figure 3) and is representative of blots hybridized with probes B, C, and D. The ∼7-kb PF6 transcript is indicated (arrow). The slight upward shift in transcript migration in the pf6-2 rescue lanes appears to result from differences in the loading of these lanes relative to the others. (B) The same Northern blot shown above was rehybridized with a probe for the CRY1 gene, which encodes the ribosomal S14 protein subunit (Nelson et al., 1994), as a control for loading of the RNA samples.