Figure 1.
HSQC spectral changes of selected Ig19-21 residues (A, C, E and G), free (Black) and bound (Red) to integrin β7 peptide and migfilin peptide (protein:peptide is 1:4). Panels B, D, F and H show the chemical shift changes of the same residues in Ig21 and Ig19 only proteins (protein:peptide ratio is 1:2). All the spectra were recorded at 600MHz at 30°C. Full spectra are shown in figure 6A and B of the supporting information.