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. Author manuscript; available in PMC: 2012 May 17.
Published in final edited form as: Biochemistry. 2011 Apr 22;50(19):3879–3890. doi: 10.1021/bi101702c

Figure 8.

Figure 8

Identification of the cardiolipin molecular species in the four-subunit CcO crystals from CL(+) (169WT) and CL(−) (169CL3) R. sphaeroides using CID MS/MS in negative ion mode on a MALDI-linear ion trap mass spectrometer, showing the marked depletion of CL(18:1)4 in CL(−) crystals compared to CL(+) crystals (A and B) as demonstrated by comparison of the absolute ion abundance of m/z 699 (top left of panels A and B ), the predominant product ion generated by CL(18:1)4 in MS/MS, corresponding to the phosphatidyldioleoylglyceride moiety. The figure identifies multiple CL species in CL(+) CcO crystals (B, C, D) but no CL (18:2)4 at m/z 1448 is seen (see also Figure 7A).