Figure 1.
mAb13 is protective against V. cholerae infection using the infant mouse cholera model. (A) Western blot of O395 V. cholerae culture supernatants following growth in TCP-inducing conditions, probed with mAb13 (top panel). The same supernatants were probed with an antibody against another secreted protein, GbpA 18, as a loading control to show that cell levels were comparable for each sample. (B) Kaplan-Meier survival curves represent mouse survival after challenge with V. cholerae mixed with purified IgG from polyclonal antiserum (25 mg/ml), mAb13 (2 mg/ml) or PBS. Six mice were inoculated per group. Data are representative of three independent experiments.