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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Anal Chem. 2011 Apr 6;83(9):3572–3580. doi: 10.1021/ac200317z

Figure 2.

Figure 2

(a) Initial validation of DNA -antibody conjugates showing orthogonal antibody loading and orthogonal antigen response. First B′-anti-AFP-B491M was added followed by F′-anti-PSA-5A6. Then 1 μg/mL of AFP was added followed by 1 μg/mL PSA. The red trace shows the response of a microring functionalized with DNA strand B and the blue trace shows the response of a microring functionalized with DNA strand F. For reference, an arrow is positioned at the time points of injection and asterisks (*) indicate the switch to running buffer. (b) Shift in resonance wavelength for F3′-anti-PSA-5A6-presenting microring resonators to 1 μg/mL PSA versus the amount(in units of Δpm)of F3 ′-anti-PSA-5A6 originally loaded onto the sensors before antigen interaction. The slope of the DNA -encoded antibody loading versus antigen response is ~2.5-foldgreater than that for covalently bound antibody, indicating an increased ability to bind to antigen.