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. Author manuscript; available in PMC: 2012 May 17.

Published in final edited form as: Biochemistry. 2011 Apr 19;50(19):3903–3912. doi: 10.1021/bi200083d

A) Overlay of R_P and S_P hybrid structures, color coded according to chemical shift differences between the two modified hybrids. R_P and S_P hybrids aligned by all heavy atoms (except boron). Nucleotides (Σ (|H1′| |H2′1| |H2′2| |H3′| |H4′| |H6/H8| |¹³C1′| |¹³C6/¹³C8 |) > 1.00 ppm, 1.00 ppm < > 0.50 ppm, < 0.50 ppm. B) R_P and S_P hybrid chemical shift difference plotted by residue.

¹H (top) closed box denotes Σ (|H1′| |H2′1| |H2′2| |H3′| |H4′|), ¹³C (middle), ³¹P (bottom) all Δ ppm. T₅–P–G₆ (³¹P) display the largest difference in chemical shift between the two modified hybrids (Δ 1.2 ppm).

Inline graphic — A) Overlay of R_P and S_P hybrid structures, color coded according to chemical shift differences between the two modified hybrids. R_P and S_P hybrids aligned by all heavy atoms (except boron). Nucleotides (Σ (|H1′| |H2′1| |H2′2| |H3′| |H4′| |H6/H8| |¹³C1′| |¹³C6/¹³C8 |) > 1.00 ppm, 1.00 ppm < > 0.50 ppm, < 0.50 ppm. B) R_P and S_P hybrid chemical shift difference plotted by residue.

¹H (top) closed box denotes Σ (|H1′| |H2′1| |H2′2| |H3′| |H4′|), ¹³C (middle), ³¹P (bottom) all Δ ppm. T₅–P–G₆ (³¹P) display the largest difference in chemical shift between the two modified hybrids (Δ 1.2 ppm).