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. Author manuscript; available in PMC: 2012 May 17.
Published in final edited form as: Biochemistry. 2011 Apr 19;50(19):3903–3912. doi: 10.1021/bi200083d

Figure 8.

Figure 8

A) Overlay of RP and SP hybrid structures, color coded according to chemical shift differences between the two modified hybrids. RP and SP hybrids aligned by all heavy atoms (except boron). Nucleotides (Σ (|H1′| |H2′1| |H2′2| |H3′| |H4′| |H6/H8| |13C1′| |13C6/13C8 |) Inline graphic > 1.00 ppm, 1.00 ppm < Inline graphic > 0.50 ppm, Inline graphic < 0.50 ppm. B) RP and SP hybrid chemical shift difference plotted by residue.

1H (top) closed box denotes Σ (|H1′| |H2′1| |H2′2| |H3′| |H4′|), 13C (middle), 31P (bottom) all Δ ppm. T5P–G6 (31P) display the largest difference in chemical shift between the two modified hybrids (Δ 1.2 ppm).