Skip to main content
. Author manuscript; available in PMC: 2012 May 24.
Published in final edited form as: Biochemistry. 2011 Apr 28;50(20):4350–4359. doi: 10.1021/bi200232c

Figure 3.

Figure 3

Multiple-turnover glycosylase activity of AlkA. (A) Representative data for AlkA-catalyzed excision of Hx from 19u (●), 19d (○), and the symmetric 25mer (■) with 10 nM AlkA and 1000 nM DNA. (B) The concentration dependence is shown for 25 (■) and 19u (●) I•T substrates. Each data point is the average of at least 3 independent determinations and the error bars indicate one standard deviation. The lines indicate the best fit to the Michaelis-Menten equation (eq 5). The kcat and KM values are given in Table 2.