Table 1.

Sequences of Oligonucleotide Substrates

25	5′ -(FAM) CGATAGCATCCTXCCTTCTCTCCAT-3′
	3′ -GCTATCGTAGGATGGAAGAGAGGTA-5′
19u	5′ -(FAM) CATCCTXCCTTCTCTCCAT-3′
	3′ -GTAGGATGGAAGAGAGGTA-5′
19d	5′ -(FAM) CGATAGCATCCTXCCTTCT-3′
	3′ -GCTATCGTAGGATGGAAGA-5′

Oligonucleotides were annealed to give the indicated duplex substrates containing a single lesion site (X). The DNA tested includes undamaged adenosine (A), the lesions inosine (I) and 1,N⁶-ethenoadenosine (εA), the abasic product, and the transition state analog pyrrolidine (Y).