TABLE 2.
Results and Parameters of Stereological Neuron and Punctae Counts in the Dentate Gyrus
| Nissl-stained hilar neurons |
Nissl-stained granule cells |
GAD-positive neurons1 |
Gephyrin- immunoreactive punctae2 |
|
|---|---|---|---|---|
| Results | ||||
| Control | 47,300 ± 2200 | 1.10 ± 0.06 (×106) | 34,000 ± 1,900 | 1.99 ± 0.13 (×109) |
| 5 d after status epilepticus | 21,500 ± 2600 | 0.88 ± 0.03 (×106) | 23,900 ± 1,800 | 1.40 ± 0.16 (×109) |
| Epileptic | 36,800 ± 3600 | 1.14 ± 0.09 (×106) | 24,100 ± 1,100 | 2.50 ± 0.17 (×109) |
| Parameters | ||||
| Sections/rat | 11.1 ± 0.2 | 11.1 ± 0.2 | 11.1 ± 0.2 | 9.0 ± 0.2 |
| Counting grid size (μm) | 100 × 100 | 200 × 200 | 100 × 100 | 400 × 400 |
| Counting frame size (μm) | 40 × 40 | 10 × 10 | 40 × 40 | 9.2 × 9.2 |
| Dissector height | Section thickness | Section thickness | Section thickness | 2.0 μm |
| Cells or punctae counted/rat | 507 ± 41 | 224 ± 10 | 248 ± 20 | 3230 ± 373 |
| Coefficient of variation | 0.308 | 0.164 | 0.218 | 0.286 |
| Mean coefficient of error3 | 0.085 | 0.050 | 0.058 | 0.046 |
Values indicate mean ± SEM unless specified otherwise. Nissl- and GAD-stained sections were from one set of animals: n = 6, 3, and 5 rats for controls, 5 days after status epilepticus, and epileptic rats. Gephyrin data were obtained from a different set of rats: n = 6/group. Please refer to Figures 2 and 4 for results of statistical tests.
1
Entire dentate gyrus (hilus, granule cell layer, and molecular layer) analyzed.
2
Regions analyzed included granule cell layer and molecular layer, not hilus.
3
Calculated according to West et al. (1991).