TABLE 3.
Parameters of Stereological Electron Microscopic (EM) Synapse Counts in the Dentate Gyrus
Thick sections/rat | 11.2 ± 2 |
EM sample sites/rat | 6 |
Counting frame size (μm) | 3.69 × 5.11 |
Dissector height (# ultrathin sections) | |
Granule cell layer | 150 |
Inner third molecular layer | 100 |
Outer two-thirds molecular layer | 100 |
No. ultrathin sections/thick section | 813 ± 26 |
GABA-positive synapses counted/rat | 1167 ± 103 |
GABA-negative synapses counted/rat | 3073 ± 184 |
GABA-negative synapse sizes measured/rat | 226 ± 12 |
Values indicate mean ± SEM unless specified otherwise. Data were collected from 14 rats: six controls, three at 5 days after status epilepticus, and five epileptic animals.