Fig. 3. H2O2 yield and pyruvate oxidase mRNA expression by wild-type and msrA mutant strains.
Bacteria were cultured in FMC medium at 37°C with 5% CO2 until stationary phase. A. The concentration of H2O2 released into the cell-free spent medium was detected by the o-dianisidine/horseradish peroxidase (Nunez de Kairuz et al., 1988) and expressed relative to the OD620 nm of cells. B. The mRNA expression level was detected by real-time PCR and reported relative to 16s rRNA expression. The data shown are the means ± SEM for 3 experiments. The means were significantly different, *p < 0.01.