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. 2011 Apr 21;8:27. doi: 10.1186/1742-4690-8-27

Figure 3.

Figure 3

Effect of HIV-1 integrase Q168L mutant on the chromatin binding activity of LEDGF/p75. Immunoblots show the effect of transient expression of HIV integrase WT and Q168L mutant on the chromatin binding strength of LEDGF/p75 WT (a) and LEDGF/p75 ΔPWWP (b), and the effect of HIV-1 integrase WT on the chromatin binding strength of LEDGF/p75 ΔIBD (c). LEDGF/p75 was detected with an anti-FLAG Mab. Immunoblots in (d) show the level of expression of HIV integrase WT and Q168L in cells coexpressing LEDGF/p75 WT, LEDGF/p75 ΔPWWP, and LEDGF/p75 ΔIBD analyzed in the experiments represented in (a), (b), and (c), respectively. HIV-1 Integrase was detected with an anti-Myc Mab. T represents a total cellular lysate.