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. 2011 Apr 21;8:27. doi: 10.1186/1742-4690-8-27

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Copyright ©2011 Astiazaran et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HIV-1 integrase promotes binding of LEDGF/p75 ΔPWWP to mitotic chromosomes. (a) Immunofluorescence analysis of LEDGF/p75-deficient cells stably expressing eGFP-tagged HIV-1 integrase and transiently transfected with FLAG-tagged LEDGF/p75 WT (panel ii) or ΔPWWP (panel i). LEDGF/p75 was detected with an anti-FLAG Mab, DAPI was used for detection of chromatin. (b) Integrase-to-chromatin tethering assay. LEDGF/p75-deficient HEK293T cells stably expressing eGFP-tagged integrase were transiently transfected with LEDGF/p75 WT (panel i) or the mutants ΔIBD (panel ii) and ΔPWWP (panel iii) and the subcellular distribution of eGFP-integrase determined by fluorescence microscopy analysis.