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. 2011 May 19;7(5):e1001386. doi: 10.1371/journal.pgen.1001386

Figure 2. Nine deletions show reduced [PSI +] appearance.

Figure 2

Genes were disrupted in the 74D-694 [PIN +] strain background. Deletion strains, transformed with the Sup35PD-GFP plasmid, were grown in 10 ml of plasmid selective media supplemented with copper for 24 hours at 30°C and plated on SD-Ade in order to determine [PSI+] induction frequency. Induction frequency and standard error were calculated with at least one transformant from each independent knock out (see Table S3) for a total of three transformants per deletion. The exception was pre9Δ which was calculated from three transformants from a single independent knock out. Only deletion strains that showed a significant difference in induction frequency compared to wildtype strains (p<0.05 unpaired t-test) were included in the figure.