Table 2. Properties of deletions that inhibit de novo induction of [PSI ⁺].

Deletion	Toxicity with Sup35PD over-expression [46]	Reaches stationary phase after 24 hours	Maintains [PIN +]	Maintains [PSI +]	[PSI +] induction	Detection of Sup35PD-GFP rings	Viability of ring cells	103Q bright aggre-gates	Growth of [PIN +] 103Q xpressing cells	Function of gene	Mammalian homolog
Wildtype	N/A	+	+	+	+	+	+ (35% ±4.4)	+	+/-	N/A	N/A
bug1Δ	enhanced	+	+	+	- - -	+	++ (59% ±6.1)	-a	- -	Involved in vesicle trafficking in anterograde ER to Golgi transport	GM130 [74]
bem1Δ	reduced	+	+	+	-	+	+ (47% ±6.0)	-a	- -	Activates Cdc42 to polymerize actin for bud formation [75], [76]	None
arf1Δ	reduced	+	+	+	-	+	+/- (22% ±4.2)	-a	-	Initiates actin assembly on golgi membranes to drive retrograde transport [77]	Arf1 [78]
hog1Δ	enhanced	+	+	+	- - -	+	+ (44% ±2.5)	-a	-	Associated with osmoregulation and possibly involved in actin polymerization in the presence of low pH [79]	MAPK p38 [80]
las17Δ	unknown	+/-	+	+	- -	- -	unknown	- - -b	+	Actin assembly factor at endocytic sites	Wiscott-Aldrich syndrome protein [81]
vps5Δ	unknown	+	+	+	- - -	-	- (13% ±2.6)	- - -	+	Actin bundling protein at endocytic sites	SNX1 [82]
sac6Δ	no effect	+	+	+	- - -	+/-	unknown	- - -	+	Vesicle trafficking in endosome to golgi	Fimbrin [83]

All strains contain the [PIN ⁺] prion. Deletion strains were assessed compared to wildtype. (-) indicates a lower degree compared to wildtype. Whether strains exhibited enhanced or reduced toxicity during Sup35PD overexpression in Tyedmers et al. [46] is listed under ‘Toxicity with Sup35PD overexpression’. ‘Reaches stationary phase after 24 hours’ indicates which deletion strains containing Sup35PD-GFP, when induced with copper for 24 hours, reach stationary phase (Figure S3). Strains carrying a deletion of LAS17 took 36 hours to reach stationary phase. ‘Maintains [PIN ⁺]’ and ‘Maintains [PSI ⁺]’ indicate strains that can propagate the respective prion after cytoduction. ‘[PSI ⁺] induction’ indicates the relative induced frequency of [PSI ⁺] formation (Figure 2). ‘Detection of Sup35PD-GFP rings’ indicates the fraction of cells containing rings after 24 hours of Sup35PD-GFP overexpression (Figure 3A), whereas the ‘viability of ring cells’ refers to toxicity associated with ring containing cells (Figure 3B). The frequency of cells with bright 103Q-GFP aggregates accumulating after one to two hours of induction (Figure 4B) are summarized under “103Q bright aggregates.” The effect of 103Q-GFP overexpression on the growth of deletions strains are under ‘Growth of [PIN ⁺] 103Q-GFP expressing cells’.

^aaggregates were reduced, but faint aggregates on diffuse background were increased.

^borted previously by [52].