Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May 19;6(5):e14813. doi: 10.1371/journal.pone.0014813

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Ye et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Guinea pigs were immunized as described in Figure 3A and sera collected at 2 weeks after the fourth immunization were analyzed for HIV neutralizing activity using a pseudovirion neutralization assay as described in Materials and Methods. HIV pseudovirions were incubated with individual serum samples from vaccinated guinea pigs at 1∶50 dilutions in 10% FCS-DMEM for 1 hr at 37°C and then added to TMZ-bl cells seeded in a 96-well plate. Two days after infection, the medium was removed and the cells were fixed and stained for β-galactosidase expressing cells. Neutralizing activity was expressed as the percentage of reduction in the number of β-galactosidase expressing cells in sample wells compared to those in control wells (% neutralization). Group 1, HA-C14S/gp41 DNA; Group 2, HA-C14S/gp41 VLP; Group 3, SHIV 89.6 VLP; Group 4, SIV Gag VLP. A. Neutralization of HIV 89.6 pseudovirions; B, Neutralization of HIV SF162 pseudovirions; C, Neutralization of HIV PVO.4 (SVPB11) pseudovirions; D, Neutralization of HIV AC10.029 (SVPB13) pseudovirions; E, Neutralization of HIV PRHPA4259.7 (SVPB14) pseudovirions.