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. 2011 May 19;6(5):e19509. doi: 10.1371/journal.pone.0019509

Figure 1. The VP16 or GAL4 activation domains (ADs) can partially complement the endogenous ADs of AvrBs3 and Hax3 in planta.

Figure 1

(A) Schematic drawing of reporter construct and co-transfection experiment. Target AvrBs3 and Hax3 boxes were inserted upstream of the minimal Bs4 promoter into a GUS reporter vector [7]. GUS reporter and constructs driving expression of TAL genes were co-delivered via Agrobacterium into N. benthamiana leaf cells. (B) Specific induction of the AvrBs3 box by AvrBs3, an AD deletion mutant (ΔAD), the ΔAD mutant complemented with the VP16-AD (VP16), and the ΔAD mutant complemented with the GAL4-AD (GAL4). (C) Specific induction of the Hax3 box by Hax3 and AD-derivatives. (B, C) GFP and constitutively expressed GUS served as negative and positive controls, respectively. Samples were taken 2 dpi (error bars indicate SD; n = 3). GUS activity was measured with MUG (4-methylumbelliferyl-β-D-glucuronide) as substrate. 4-MU, 4-methylumbelliferone.