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. 2011 May 19;7(5):e1002057. doi: 10.1371/journal.ppat.1002057

Figure 2. MARCH5 synergizes TLR7- induced NF-κB activation.

Figure 2

A and B, Equal amounts of the indicated Myc-tagged MARCH5 constructs were transfected into Raw264.7 cells together with NF-κB (A) or PRDIII-I reporter plasmids (B). Twenty-four hours after transfection, cells were stimulated with R837 (10 µg/ml) or LPS (100 ng/ml) for 6 h before luciferase assays were performed. C and D, induction of IL6, TNFα, and ISG15 mRNA by R837 (C) or LPS (D) stimulation in the presence of the indicated plasmids in RAW264.7 cells were measured by quantitative PCR. E, BMDCs (bone marrow-derived dendritic cells) were transfected with the indicated plasmids and then stimulated by R837 (10 µg/ml). Induction of IL6, TNFα, and ISG15 mRNAs was measured by qPCR. Data from A–E are presented as means ± S.D. from three independent experiments. *, P<0.05; **, P<0.01. WT, MARCH5 wild type; C2A, MARCH5 C65, 68A mutant; and ΔR, MARCH5 RING domain deletion mutant.