Figure 3. Plant PA levels increase in response to treatment with P. indica exudate.

Five-days old seedlings were ³²P_i-labelled overnight and then treated with P. indica exudates. (A) Time series of plant PA amounts induced by 50 µl P. indica exudates. (B) Dose response curve of plant PA production in response to different amounts of P. indica exudate. Lipids were extracted, analysed by thin layer chromatography and PA levels were quantified by phosphoimaging. ³²P-PA control levels were ∼1.5% of the total ³²P-labelled lipids. The values represent: radioactivity [+P. indica extract/+buffer]. Bars represent SEs, based on 3 independent experiments. Bars marked with an asterisk are significantly different compared to wild type (p<0.05).