Table 4. Identification of the bifidobacterial DGGE band positions by sequencing and the incidence of the bands in secretor (14) and non-secretor samples (57).
Best Blast hit (best cultured hit, similarity) A | Band positionB | Detected bands | % of non-secretors | % of secretors |
B. longum | 53.5% | 56 | 79 | 79 |
Uncultured bifidob. (B. adolescentis, 99%) | 62.2% | 41 | 50 | 60 |
B. adolescentis | 26.6% | 40 | 14 | 67** |
not sequenced | 17.7% | 18 | 0 | 32** |
B. catenulatum/pseudocatenulatum | 63.7% | 18 | 0 | 32** |
B. bifidum | 29.7% | 17 | 7 | 28 |
not sequenced | 20.4% | 16 | 7 | 26 |
B. adolescentis | 22.3% | 13 | 14 | 19 |
Uncultured bifidob. (B. adolescentis/ruminantium, 98–99%) | 43.8% | 13 | 14 | 19 |
Uncultured bifidob. (B. catenulatum, 99%) | 47.3% | 9 | 7 | 14 |
Uncultured bifidob. (B. adolescentis, 99%) | 55.0% | 9 | 7 | 14 |
Uncultured bifidob. (B. ruminantium, 99%) | 44.5% | 8 | 7 | 12 |
Not sequenced | 39.3% | 7 | 7 | 11 |
The similarity of the best Blast hit for a cultured strain is shown in parentheses, in cases where an uncultured bacterium was the best hit. Detailed data for the identification of each position is shown in table S1. B Only the band positions that were detected at least in 10 % of the samples are shown.** Significant differences: Band positions 26.6%, 17.70% and 63.7% were more frequently detected in secretor than non-secretor samples (Fisher's exact test, p<0.01), and in Lewis b than Lewis a samples (Fisher's exact test, p<0.01 for 17.70% and 26.60% ; p<0.02 for 63.70%).