Figure 5. Mutations in the ATPase domain and in the SBD subvert the PfHsp70-1-mediated improvement of growth in the ssa1-45 or ssa1Δssa2Δ mutants.

(A) The indicated mutant strains transformed with the designated plasmids were grown to log phase, serially diluted, and plated on selective media and incubated at the indicated temperatures for 2–3 d. (B) Cells were plated on selective media containing the indicated stress-inducing agent, grown at 26°C, and imaged after 3 d. In both panels, the images shown are representative of at least three independent experiments, all demonstrating the same phenomena. “-” indicates an empty vector control.