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. 2011 May 19;6(5):e19998. doi: 10.1371/journal.pone.0019998

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Tayebi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PRIOC mAbs failed to stain prion aggregates in N2a and prn-p^0/0 glial cells. (A). Staining with PRIOC4 did not display PrPSc oligomer-specific stain in prn-p^0/0 glial cells with no permeabilization. (B). Staining with PRIOC4 did not display PrPSc oligomer-specific stain in N2a cells with no permeabilization. (C). Staining with PRIOC4 did not display PrPSc oligomer-specific stain in prn-p^0/0 glial cells following permeabilization. (D). Staining with PRIOC4 did not display PrPSc oligomer-specific stain in N2a cells following permeabilization. Florescence microscopy was performed and images from each source [FITC (450–490 nm), Texas red (510–560 nm) and DAPI (330–380 nm)] were collected. Scale bar = 25 µm.