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. 2011 May 19;6(5):e19998. doi: 10.1371/journal.pone.0019998

Figure 8. Immunodetection of rPrP oligomers/multimers with PRIOC mAbs by Sandwich ELISA.

Figure 8

(A). Oligomers derived from rPrP monomers were used to assess specific binding of PRIOC mAbs. 50 µl of 5 µg/ml of PRIOC was used to coat the ELISA plate in coating buffer. rPrP-derived oligomers were added to the wells followed by a biotinylated PRIOC mAb. (B). Fibrils derived from rPrP monomers (used to produce the oligomers) were assayed with thioflavin T fluorescence (Tht). (C). This panel represents a combination of panel (A) and panel (B) and demonstrates the dynamic of oligomer/fibril formation of monomeric rPrP as assessed by the PRIOC mAbs and Tht. Error bars represent the mean level derived from n = 4 wells.