Figure 9. Immunodetection of Aβ peptide and α-synuclein oligomers/multimers with PRIOC mAbs by Sandwich ELISA.

Oligomers derived from Aβ peptide and α-synuclein monomers were used to assess specific binding of PRIOC mAbs. 50 µl of 5 µg/ml of PRIOC was used to coat the ELISA plate in coating buffer. Aβ peptide or α-synuclein-derived oligomers were added to the wells followed by a biotinylated PRIOC mAb. Error bars represent the mean antibody level derived from n = 4 wells.