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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: J Immunol. 2011 Feb 16;186(6):3606–3614. doi: 10.4049/jimmunol.1003470

Figure 3.

Figure 3

Serum bactericidal responses of wild-type mice to fHbp vaccines. Titers of individual mice immunized with different vaccines are represented by symbols (fHbp WT vaccine, circles; R41S mutant vaccine, triangles; and E218A/E239A mutant vaccine, + symbols). The geometric mean titers are indicated by horizontal lines. In an initial study (left), wild-type mice were immunized with three doses of fHbp WT or R41S mutant vaccines. As a control, a group of mice were immunized with a second mutant fHbp vaccine, E218A/E239A, which in previous studies showed lack of fH binding but had impaired immunogenicity in wild-type mice (30). Mice immunized with the R41S mutant developed similar geometric mean bactericidal titers as those immunized with the wild-type fHbp (71 vs. 77, respectively, P=0.32), whereas mice immunized with the E218A/E239A mutant had a GMT of 9 (P=0.01, compared to wild-type fHbp vaccine). The similar immunogenicity of the wild-type fHbp and R41S vaccines was confirmed in a replicate study, which is shown on the right (GMTs of 115 vs. 83, respectively, P=0.90).