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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: J Immunol. 2011 Feb 16;186(6):3606–3614. doi: 10.4049/jimmunol.1003470

Figure 8.

Figure 8

Serum antibody responses of human fH transgenic mice immunized in Study 2 with a mutant fHbp (R41S) vaccine that did not bind fH. A, IgG anti-fHbp responses. Titers of individual mice are represented by symbols (control fHbp WT vaccine, circles; R41S mutant vaccine, triangles; Al(OH)3 control, filled diamonds). The geometric mean titers are indicated by horizontal lines. The symbols for mice immunized with negative control vaccine are from testing serum pools (each pool from 3-4 individual mice). Comparing respective IgG GMT of wild-type fHbp and R41S mutant vaccine groups, P=0.96. B, Serum bactericidal responses measured with human complement against group B strain H44/76. Comparing respective SBA GMT of wild-type fHbp and R41S mutant vaccine groups, P=0.11. When the analysis of the SBA responses was adjusted by general linear regression for the confounding inverse effect of serum human fH concentrations on SBA responses of Tg mice immunized with the fHbp that bound fH, the higher responses to the mutant R41S vaccine were significant (P=0.018, See Figure 9 and text).