Table 1. Bacterial strains and plasmids used in the study.
| Strain or plasmid | Genotype and description * | Reference or source |
| Salmonella | ||
| S. TyphimuriumSL1344 | wild-type Smr xyl hisG rpsL | ATCC |
| OG2011 (phoP ssrB) | SL1344 phoP::Tn10 Tcr ssrB::kan transduced by P22 from ssrB | This study |
| phoP | SL1344 phoP::Tn10 Tcr | [58] |
| ssrB | SL1344 ssrB::kan transduced by P22 from 14028s | [59] |
| S. bongori SARC 12 | [60]M. Hensel lab | |
| DE1.10.3 (phoP) | S. bongori SARC12Δ phoP | This study |
| E. coli | ||
| BL21(DE3) | F- ompT hsdSβ(rβ-mβ-) dcm gal (DE3) tonA | Lab collection |
| DH5α | F- ϕ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk−, mk+) supE44 thi-1 gyrA96 relA1 λ- | Lab collection |
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km λpir | Lab collection |
| TOP10 | mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZ ΔM15 ΔlacX74 deoR recA1araD139 Δ(ara, leu) 7697 galU galK λ- rpsL endA1 mupG | Invitrogen |
| Plasmids | ||
| pACYC184 | Tcr Cmr cloning vector | NEB |
| pCR-Blunt | Kanr cloning vector | Invitrogen |
| pDE-PhoPQSB | S. bongori phoPQ cloned into pACYC184 | This study |
| pDE-PhoPQSB169V | S. bongori phoP haboring V169 cloned into pACYC184 | This study |
| pDE-PhoPQST | S. Typhimurium phoPQ cloned into pACYC184 | This study |
| pDE-SsrABST | S. Typhimurium ssrAB cloned into pACYC184 | This study |
| pET-28a(+) | Kanr cloning vector for N-terminal His tag fusions | Novagen |
| pRE112 | pGP704 suicide plasmid; pir dependent; sacB | [55] |
| pMC1403 | Ampr lacZY cloning vector | [30] |
| pOG-His-PhoP | N-terminus 6-His tag of PhoP in pET28-a | This study |
| pOG-pagO-lacZ | pagO fused to lacZ in pMC1403 | This study |
| pOG-mig5-lacZ | fkpA fused to lacZ in pMC1403 | This study |
| pOG-PCR-sseL-RACE | RLM-RACE of sseL cloned into pCR-Blunt | This study |
| pOG-sseL-lacZ | sseL fused to lacZ in pMC1403 | This study |
| pOG-sseL-lacZ-S1 | S1 mutation in sseL promoter fused to lacZ in pMC1403 | This study |
| pOG-sseL-lacZ-S2 | S2 mutation in sseL promoter fused to lacZ in pMC1403 | This study |
| pOG-sseL-lacZ-S1+2 | S1+S2 mutations in sseL promoter fused to lacZ in pMC1403 | This study |
| pOG-ybjX-lacZ | ybjX fused to lacZ in pMC1403 | This study |
| pUC19 | Ampr high-copy number cloning vector | NEB |
*Sm, streptomycin; Cm, chloramphenicol; Kan, kanamycin.