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. 2011 May 19;6(5):e20188. doi: 10.1371/journal.pone.0020188

Figure 7. Knockdown of importin-β1 inhibits STAT3-GFP nuclear import.

Figure 7

HeLa cells were transfected with vimentin siRNA (control) or importin-β1 siRNA and STAT3-GFP. A) Images of STAT3-GFP localization. Four independent experiments indicated 100% of cells expressing control siRNA had prominent nuclear STAT3-GFP. In cultures expressing importin-β1 siRNA, an average of 58% of cells showed a defect in STAT3-GFP nuclear import, with 18% of cells showing the severe impairment indicated in the image. B) Effective knockdown of importin-β1 mRNA (Imp-β1) relative to GAPDH mRNA measured in cells expressing importin-β1 siRNA or vimentin siRNA (CTRL) by RT-PCR. Average knockdown of importin-β1 mRNA from four experiments was approximately 56% measured with Image J software. C) Knockdown of importin-β1 protein relative to tubulin was approximately 50% evaluated by Western blot.