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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Mar 31;31(6):1387–1396. doi: 10.1161/ATVBAHA.110.222547

Figure 5. Oxidative stress-stimulated VSMC promote BMM migration in a Runx2/RANKL-dependent manner.

Figure 5

A) After VSMC were cultured in osteogenic media for 2 weeks with or without 0.4 mM H2O2, cells were coated on the lower side of Transwell filter, and DiI-labeled BMMs were added to the upper chambers. After 24 hours, migrated BMMs were measured using a microplate fluorescence reader. Results from 3 independent experiments performed in duplicate are shown (p = NS for unstimulated WT VSMC vs. serum-free, p = 0.01 for oxidative stress-stimulated WT VSMC vs. serum-free). B) WT VSMC were pre-incubated in osteogenic media for 2 weeks with or without 0.4 mM H2O2, then coated on the lower side of Transwell. Effects of conditioned media from the pre-incubation or addition of OPG (50ng/ml) to the upper side of the Transwell on macrophage migration was determined. Results from 3 independent experiments performed in duplicate are shown (*p = 0.005 for oxidative stress-stimulated VSMC vs. serum-free control). Addition of OPG abolished the effects.