Fig. 2.

Hydrolysis of human LDL by recombinant human sPLA₂-III, -V and –X in vitro. After LDL (1 mg/ml) was incubated with or without 10 or 50 nM sPLA₂ for 4 h at 37°C, lipids were extracted and applied to ESI-MS (4000Q TRAP; Applied Biosystems), as described previously [74, 75]. Representative results of ESI-MS for choline-containing phospholipids on a positive ion mode (a) and lysophospholipids on a negative ion mode (b) are shown. Major peaks of PC, LPC, LPE and SM molecular species are indicated. Asterisks indicate the peaks that were significantly changed by addition of each sPLA₂. Top-right graphs show quantified data of LPC and LPE. For more details, please see [28]