Table 2.
Recoveries (%) and relative standard deviations RSD (%) obtained by SPE-HPLC/MS analysis of three different food products fortified with a standard solutions of nine intense sweeteners at three spiking levels (50%, 100% and 125% of maximum usable dose, MUD)
| Analyte | MUDa | Recovery (RSD) [%] spiking level | ||
|---|---|---|---|---|
| 50% MUD | 100% MUD | 125% MUD | ||
| Cola drink | ||||
| ACS-K | 350 | 100.7 (1.3) | 98.4 (1.1) | 103.6 (1.8) |
| SAC | 80 | 95.3 (1.3) | 89.9 (2.7) | 92.9 (1.4) |
| CYC | 250 | 95.5 (0.7) | 95.7 (2.1) | 97.1 (0.8) |
| ASP | 600 | 88.5 (0.6) | 88.7 (2.2) | 92.3 (0.7) |
| SCL | 300 | 94.4 (0.4) | 93.5 (2.1) | 96.6 (0.3) |
| DUL | 20b | 99.8 (0.4) | 105.7 (1.8) | 106.2 (3.4) |
| ALI | 20b | 91.3 (5.3) | 90.2 (3.6) | 94.1 (3.2) |
| NHDC | 30 | 103.9 (0.9) | 96.5 (1.7) | 99.3 (0.4) |
| NEO | 20 | 95.3 (1.0) | 92.7 (1.4) | 96.2 (0.4) |
| Yoghurt | ||||
| ACS-K | 350 | 96.4 (1.5) | 97.5 (1.3) | 98.8 (0.4) |
| SAC | 100 | 86.3 (0.2) | 87.4 (0.8) | 85.7 (1.0) |
| CYC | 250 | 101.7 (2.5) | 98.3 (3.9) | 96.3 (0.6) |
| ASP | 1000 | 86.2 (1.2) | 88.4 (2.9) | 99.7 (1.8) |
| SCL | 400 | 90.6 (1.4) | 93.8 (2.9) | 98.8 (0.3) |
| DUL | 20b | 99.5 (6.1) | 102.5 (3.2) | 100.2 (1.4) |
| ALI | 20b | 91.5 (1.6) | 92.1 (3.0) | 88.7 (4.0) |
| NHDC | 50 | 89.6 (1.7) | 86.5 (3.5) | 87.0 (1.6) |
| NEO | 32 | 90.7 (1.7) | 87.1 (5.0) | 97.8 (1.9) |
| Fish marinade | ||||
| ACS-K | 200 | 96.5 (3.4) | 101.1 (2.4) | 104.7 (0.7) |
| SAC | 160 | 97.3 (3.2) | 97.9 (2.4) | 100.8 (2.7) |
| CYC | 200c | 98.3 (4.4) | 88.4 (3.8) | 101.7 (2.0) |
| ASP | 300 | 85.1 (4.9) | 90.9 (3.8) | 88.9 (1.2) |
| SCL | 120 | 91.4 (7.5) | 100.2 (4.5) | 106.7 (0.3) |
| DUL | 20b | 98.7 (6.7) | 99.9 (5.0) | 105.3 (0.2) |
| ALI | 20b | 96.3 (9.2) | 99.3 (4.4) | 103.9 (0.7) |
| NHDC | 30 | 89.3 (8.7) | 85.0 (4.4) | 88.2 (2.2) |
| NEO | 20 | 87.8 (9.8) | 84.2 (4.3) | 88.4 (0.5) |
aBeverages, in milligrammes per litre, yoghurt and fish marinade, in milligrammes per kilogramme
bNot allowed for use in foodstuffs according to the EU directives; proposed value
cNot authorised for use in fish products according to the EU directives, proposed value