Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 23;38(2-2):179–190. doi: 10.1016/j.molcel.2010.04.009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 ELL & Excerpta Medica.

Open Access under CC BY 3.0 license

PMC Copyright notice

KDM2A Is Targeted to Nonmethylated DNA In Vivo

(A) Indirect immunofluoresence with anti-KDM2A antibodies (red) and DAPI staining of DNA (blue). Endogenous KDM2A staining is found throughout the nucleoplasm in Dnmt+/+ MEFs (upper left panel). DAPI bright-staining foci mark regions of densely methylated pericentromeric heterochromatin (lower left panel). In Dnmt1^−/− MEFs almost devoid of CpG methylation, KDM2A localizes to the hypomethylated pericentromeric DAPI bright-staining repeat regions (compare upper and lower right panels).

(B) A schematic indicating the epitope-tagged wild-type and mutant versions of KDM2A.

(C) Indirect immunofluorescence analyzing localization of exogenous KDM2A expressed in Dnmt1^+/+ (left panel) and Dnmt1^−/− MEFs (right panels). Deletion of the ZF-CxxC domain (ΔCxxC), but not mutation of the catalytic domain (H212A) or deletion of the PHD domain (ΔPHD), abrogates localization of KDM2A to hypomethylated pericentromeric regions in Dnmt1^−/− MEFs.