Fig. 3.

Replication of wild-type GBV-B and chimeric viruses in marmosets following intra-hepatic RNA injection. Marmosets received a total of 150 μg of either wild-type or chimeric RNAs via intra-hepatic injection. GBV-B RNA from serum was measured using quantitative RT-PCR (Taqman). (A) Titres following injection with wild-type GBV-B RNA. (B) Titres following injection with ΔNC + p7 chimeric RNA, *indicates where animal AA383 was sacrificed due to acute illness. (C) Titres following injection with ΔC + p7 RNA, ^#indicates where serum taken from animal P164 for infection of naı¨ve animals. (D) Nested RT-PCR for p13 region on RNA extracted from animal AA383 liver homogenate (left panel) or from pooled sera of the two injected animals (right panel). A positive (+ve) control using either 7.5 or 0.75 fg of in vitro transcribed chimeric RNA as template is shown for comparison.