Fig. 3.

Effects of removal of extracellular Ca²⁺ for different periods of time on the Ca²⁺ transient induced by CCh in the ECs of intact rat tail artery. (A(i–ii)) Original records of CCh induced Ca²⁺ transients in control conditions and at different times of exposure (i) 1–3 min, (ii) 30 min to Ca²⁺-free solution containing 2 mM EGTA. B(a–d) Percentage of inhibition of the initial phasic component of the Ca²⁺ transient induced by CCh after exposure of the ECs to 30 s (b), 5 min (c) and 30 min (d). Peak Ca²⁺ transient in control condition was taken for 100% (a). (c and d) P < 0.05, significantly different from control.