Table 3.
Expression of cytokines as well as iNOS and HO-1 in PBMCs as measured by flow cytometry and real-time RT-PCR.
| Cytokine | Protein level (% of positively stained cells) |
Variation of normalized mRNA expression calculated relative to group median (=1) |
|||||
|---|---|---|---|---|---|---|---|
| Mean | SD | Range | Positive/total (min. Ct) | Min | Max | Variance | |
| IL-1 | 7.1 | 3.5 | 1.8–19.0 | 32/32 (20.75) | 0.10 | 40.86 | 50.29 |
| IL-2 | 7.9 | 3.9 | 2.1–21.7 | 32/32 (29.6) | 0.03 | 11.81 | 19.98 |
| IL-4 | 7.3 | 2.5 | 4.1–13.7 | 31/32 (36.9) | 0.02 | 49.39 | 77.59 |
| IL-6 | 6.3 | 2.6 | 1.4–11.4 | 28/32 (32.6) | 0.01 | 123.79 | 823.62 |
| IL-10 | 5.5 | 1.9 | 2.3–9.3 | 32/32 (22.6) | 0.23 | 19.64 | 25.86 |
| IL-12p35 | 6.0 | 2.6 | 2.5–13.2 | – | – | – | – |
| TNF-α | 55.6 | 10.4 | 33.2–73.0 | 32/32 (22.6) | 0.15 | 1.38 | 4.8 |
| IFN-γ | 26.1 | 8.5 | 12.1–46.3 | 32/32 (28.6) | 0.16 | 82.82 | 208.41 |
| iNOS | – | – | – | 30/32 (30.7) | 0.0003 | 58.32 | 160.99 |
| HO-1 | – | – | – | 32/32 (29.9) | 0.02 | 1.64 | 4.7 |