Microcolumnar organization of double bouquet cells. (A,B,D,E) Low magnification photomicrographs of calbindin immunostained (CB-ir) sections from the human: primary somatosensory (area 3b); temporal associative (area 22); and primary (area 17) and secondary (area 18) visual areas, showing the distribution of CB-ir cell bodies and double bouquet cell horse-tails. Note the large number and the regular distribution of double bouquet cell horse-tails. (C,F) High magnification of the boxed areas shown in (B,E), respectively, to highlight the differences in thickness, density, and number of axon collaterals of CB-ir double bouquet cell horse-tails in different cortical areas. Scale bar: 150 μm in (A,B,D,E); 45 μm in (C–F). Adapted from Ballesteros-Yánez et al. (2005).