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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Endocr Relat Cancer. 2010 May 18;17(2):513–524. doi: 10.1677/ERC-09-0272

Figure 3. The L95P parafibromin mutant retains interaction with PAF1 complex components.

Figure 3

A. 24 hours after transfection with vector only (Vec), or AU5 epitope-tagged wild-type (wt) or L95P mutant parafibromin (Pfb) HeLa cells were immunoprecipitated using AU5 monoclonal antibody and both the starting cell lysate (lower) and washed immunoprecipitates (upper) were analyzed by immunoblotting using anti-parafibromin, anti-Paf1 and anti-Leo1 antibodies as indicated. B. Histograms comparing the amount of immunoreactive parafibromin, Paf1 and Leo1 in the anti-AU5 immunoprecipitates.