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Chromatin association of GINS in the presence or absence of Sld7. YYT2 (6FLAG–PSF1) or YTT9 (6FLAG–PSF1 sld7Δ) cells were arrested in G1 phase with α-factor and released at 25°C. Aliquots of the cells were sampled at 5 min intervals and subjected to flow cytometric analysis (A) and ChIP assay (B). The black and grey bars indicate the YYT2 (SLD7) and YTT9 (sld7Δ) cells, respectively.
