Table I. Cleavage sites generated by Glu-C in LM-111 and fibronectin-1.
Top, neo-N-terminal peptides from denatured LM-111 and fibronectin-1 partially digested with Glu-C. Bottom, neo-N-terminal peptides from native LM-111 and fibronectin-1 partially digested with Glu-C. Cleavage sites were identified with ATOMS. Neo-N-terminal peptides from LM-111 are presented on the left side of the table, and peptides from fibronectin are on the right. Positions of the neo-N-terminal peptides in the precursor proteins are identified by the numbers in superscript at the beginning of the peptides.
| P1 | Identified LM-111 peptides | P1 | Identified fibronectin-1 peptides |
|---|---|---|---|
| Proteolysis of denatured LM-111 and fibronectin-1 with Glu-C | |||
| α1 | |||
| Glu | 1368LCVCPPGTAGHSCQDCAPGYYR | Glu | 67RTYLGNALVCTCYGGSR |
| Glu | 1730LKAAKDLLSR | Glu | 89SKPEAEETCFDKYTGNTYR |
| Glu | 2029SAVKTLEDVLALSLR | Glu | 93AEETCFDKYTGNTYR |
| Glu | 2036DVLALSLR | Glu | 272RHTSVQTTSSGSGPFTDVR |
| Glu | 2084MQANLLLDR | Glu | 393QDQKYSFCTDHTVLVQTR |
| Glu | 2873IMVNGQQLDKDRPLSASAVDR | Glu | 566TGTFYQIGDSWEKYVHGVR |
| Glu | 2901GTFFEGSGYAALVKEGYKVR | Glu | 756LSEEGDEPQYLDLPSTATSVNIPDLLPGR |
| β1 | |||
| Glu | 33GSCYPATGDLLIGR | Glu | 1256SVPISDTIIPAVPPPTDLR |
| Glu | 172SSFPGISTGPMKKVDDIICDSR | Glu | 2227SGFKLLCQCLGFGSGHFR |
| Glu | 1006GDHCQLCQYGYYGDALR | Glu | 2311YLGAICSCTCFGGQR |
| Glu | 1099FTGQCQCMPGFGGR | Glu | 2344GTTGQSYNQYSQR |
| Glu | 1120LFWGDPDVECR | ||
| Glu | 1559VILQQSAADIAR | ||
| γ1 | |||
| Glu | 392ACSPCHCSPVGSLSTQCDSYGR | ||
| Glu | 538WSSDRQDIAVISDSYFPR | ||
| Glu | 804LCDDGYFGDPLGSNGPVR | ||
| Glu | 847CLKCIYNTAGFYCDR | ||
| Glu | 1050LESLIANLGTGDDMVTDQAFEDR | ||
| Glu | 1087AQEVKDVDQNLMDR | ||
| Glu | 1361ANDILNNLKDFDR | ||
| Proteolysis of native LM-111 and fibronectin-1 with Glu-C | |||
| β1 | |||
| Glu | 26FSYGCAEGSCYPATGDLLIGR | Glu | 619TPSQPNSHPIQWNAPQPSHISKYILR |
| Glu | 573RQYIQDRIPSWTGPGFVR | Pro | 1178TNLHLEANPDTGVLTVSWER |
| γ1 | |||
| Val | 1037KDKAAEHRVKLQELESLIANLGTGDDMVTDQAFEDR | Glu | 2197GLNQPTDDSCFDPYTVSHYAVGDEWER |
| Glu | 1589DIKKTLPTGCFNTPSIEKP | Glu | 2227SGFKLLCQCLGFGSGHFR |
| Glu | 2344GTTGQSYNQYSQR |