Fig. 2.

Heterogeneity of the glycan moieties on the four glycosylation sites of kappa-5. Kappa-5 was subjected to reduction and alkylation, digested with trypsin and the resulting glycopeptides were analyzed by LC-ion trap-MS. Mass spectra cover the four glycosylation sites N₁₁₆ (A), N₁₄₃ (B), N₁₇₄ (C), and N₂₅₁ (D). Differences in fucose, N-acetylglucosamine, and N-acetylgalactosamine content of the glycan moiety are indicated. Nonglycopeptide signals are marked with asterisks (*). Gray triangle, fucose; light gray square, N-acetylgalactosamine; dark gray square, N-acetylglucosamine; gray circle, mannose; open star, xylose.