Fig. 3.

Reaction of IgE in human infection sera with kappa-5. (A, B) Blots containing SBA-purified kappa-5 were treated (p) with metaperiodate to destroy glycan structures, or without (-). Kappa-5 is indicated by the arrowhead. A, Treatment efficiency was analyzed with a protein stain (Silver), a monoclonal antibody against kappa-5 (1G2) and SBA reactivity (SBA). B, Sera from six human subjects known to be infected with S. mansoni were used to probe the kappa-5 blots. IgE antibody reactivity was visualized by a secondary antibody specific for IgE. C, Kappa-5 was treated with β-N-acetylhexosaminidase (κ5 HexNAc), α-fucosidase from X. manihotis (κ5 Fuc) or a combination of the enzymes (κ5 FucHexNAc), and the kappa-5 variants were tested in an ELISA for their reactivity with IgE from two human infection sera. One out of two representative experiments is shown.