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. 2010 Apr;8(2):175–185. doi: 10.1089/adt.2009.0249

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© Mary Ann Liebert, Inc.

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Fig. 2. — Optimization of PEPC-MDH assay system. Data were collected under nitrogen as described in Materials and Methods. All data were collected under nitrogen in 384-well microplates. (A) Determination of the K_m apparent with respect to bicarbonate. The value obtained is consistent with literature values.^24,25 Data were fit to the Michaelis–Menten equation. (B) Determination of assay linear time ( = 0.75 mM HCO₃, ▴ = no HCO₃). Under optimized assay conditions, the bicarbonate signal was linear for ∼7 min.

Inline graphic — Optimization of PEPC-MDH assay system. Data were collected under nitrogen as described in Materials and Methods. All data were collected under nitrogen in 384-well microplates. (A) Determination of the K_m apparent with respect to bicarbonate. The value obtained is consistent with literature values.^24,25 Data were fit to the Michaelis–Menten equation. (B) Determination of assay linear time ( = 0.75 mM HCO₃, ▴ = no HCO₃). Under optimized assay conditions, the bicarbonate signal was linear for ∼7 min.