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. 2010 Dec 28;19(5):990–999. doi: 10.1038/mt.2010.289

Figure 2.

Figure 2

Generation of immunological responses upon vaccination with in vitro transcribed mRNA; exemplary interferon-γ (IFN-γ) enzyme-linked immunosorbent spot (ELISpot) assays: course of several tumor-associated antigen (TAA). Autologous peripheral blood mononuclear cells from human leukocyte antigen (HLA)-A2+ patients were pulsed with the peptides MUC 1.1, MUC 1.2, MAGE, CEA, survivin 1 and 2 deduced from TAA used in the vaccine and used as stimulators. (a–c) Representative ELISpot assays of several different T cell response patterns of three patients. Samples from prevaccination and representative samples after one or more vaccinations were evaluated simultaneously. CEA, carcinoembryonic; MAGE, melanoma-associated antigen 1; MUC 1, mucin 1.