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. 2010 Dec 28;19(5):990–999. doi: 10.1038/mt.2010.289

Figure 4.

Figure 4

Lytic activity of vaccine-induced T lymphocytes. Cytotoxic T lymphocytes obtained after in vitro restimulation efficiently lysed target cells [dendritic cells (DC)] pulsed with the antigenic peptides (MUC1, survivin, MAGE, CEA), as well as DC electroporated with a mixture of RNA coding for tumor-associated antigen (TAA). Lysis was shown to be antigen-specific since no lysis was detected when target cells were loaded with the peptide derived from HIV or when electroporated with enhanced green fluorescent protein (EGFP) RNA. In addition, the human leukocyte antigen (HLA)-A2 positive tumor cell line A498 [renal cell carcinoma (RCC)], as well as HLA-A2 negative control cell lines like SK-OV-3 cells (ovarian cancer) and CaKi-2 (RCC) were included demonstrating HLA restricted lysis of CTL. K562 [chronic myelogenous leukemia (CML) in blast crisis] was used to exclude natural killer-cell mediated lysis. Data from two patients are presented (a,b: patient number 11 and c,d: patient number 8, cohort B). CEA, carcinoembryonic; MAGE, melanoma-associated antigen 1; MUC 1, mucin 1.