Skip to main content
. 2011 Feb 22;19(5):913–921. doi: 10.1038/mt.2010.303

Figure 5.

Figure 5

Visualization of the DNA/membrane interaction and fluorescence intensity distribution of the DNA aggregates in CHO cells with and without latrunculin B drug treatment. The cells were pulsed in the presence of TOTO-1 labeled plasmid DNA (pEGFP-C1) with the following parameters: 10 pulses of 5 ms at 0.4 kV/cm and 1 Hz. (a,c,e,g) control cells, (b,d,f,h) latrunculin B treated cells (0.1 µmol/l, 37 °C, 1 hour before the electric field application) (in both conditions, 34 cells were visualized), (i) number of aggregates counted as a function of the fluorescence intensity in control (gray) and treated cells (black) (number of aggregates counted n = 330 for the control cells and n = 154 for the treated cells), (i inset) mean fluorescence intensity (mean + SEM). The black arrow on the right side indicates the direction of the electric field. Bar = 20 µm. CHO, Chinese hamster ovary; EGFP, enhanced green fluorescence protein.