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. 2011 Feb 22;19(5):913–921. doi: 10.1038/mt.2010.303

Figure 6.

Figure 6

Gene expression in CHO cells treated with latrunculin B before or after electric field application. The cells were transfected with pEGFP-C1 plasmid DNA with the following parameters: 10 pulses of 5 ms at 0.4 kV/cm and 1 Hz and analyzed by flow cytometry. (a) Percentage of transfected cells, (b) transfection efficiency both normalized in relation to T. Experiments were performed in triplicate and repeated seven times (mean + SEM). NT, nontreated and nontransfected cells; E, cells exposed to electric field but without DNA; T, nontreated but transfected cells (our reference); L1hT, latrunculin B incubation performed for 1 hour before the transfection; TL1h/TL24h, latrunculin B incubation performed for 1 hour /24 hours after the transfection; CHO, Chinese hamster ovary; EGFP, enhanced green fluorescence protein.