Table 1.

Examples of nonannular lipids

Protein	Method	Lipid	Function	Selected references
β2-adrenergic receptor)	X-ray crystallography	Chol	Protein fold (putative)	Hanson et al. 2008
Caveolin-1	SDS-PAGE, in vitro oligomerization	Chol	oligomeric state	Monier et al. 1995; Murata et al. 1995
Cytochrome bc1 complex	X-ray crystallography	PI, CL	Stability and integrity of the complex, enzymatic activity	Gomez and Robinson 1999; Lange et al. 2001
Cytochrome c oxidase	X-ray crystallography	PG	Oxygen transfer (putative)	Shinzawa-Itoh et al. 2007
K+-channel	X-ray crystallography	anionic PLs	Potassium conductance	Valiyaveetil et al. 2002; Marius et al. 2008
Metabotropic glutamate receptor	Agonist binding kinetics, lipid mass spectrometry	Erg	Allosteric effector, targeting to sterol-rich microdomains	Eroglu et al. 2003
Mitochondrial ADP/ATP carrier	X-ray crystallography	CL	Oligomeric state	Nury et al. 2005
Nitrate reductase A	X-ray crystallography	PG	Oligomeric state/protein fold (“building blocks”)	Bertero et al. 2003
Oxytocin receptor	Agonist binding kinetics	Chol	Allosteric effector	Gimpl et al. 2002a
Peripheral-type benzodiazepine receptor	Ligand-dependent cholesterol uptake and release kinetics, mutational analyses	Chol	Cholesterol transport and compartmentali-zation (putative)	Li and Papadopoulos 1998; Jamin et al. 2005
Plasma membrane Ca2+-ATPase	Transport & fluorescence assays	Cer DAG	Allosteric effector	Perez-Gordones et al. 2009
Rhodopsin	In vitro photolysis assays, differential scanning calorimetry (DSC)	Chol	Metarhodopsin formation, reprotonation (photocycle)	Mitchell et al. 1990; Bennet et al. 2008
Serotonin1a receptor	Agonist binding kinetics	Chol	Allosteric effector	Pucadyil and Chattopadhyay 2004
Vacuolar-type Na+-ATPase	X-ray crystallography	DPPG, DPG	Oligomeric state/protein fold (“building blocks”)	Murata et al. 2005

For details, see text and Ernst et al. 2010.